Table 1.

Gene specific primers used in PCR Amplification.

Genes	Sequences		Length
GAPDH	Forward	5′-TGCATCCTGCACCACCAA-3′	18
	Reverse	5′-CCTTCCACAATGCCAAAGTTG-3′	21
Type I Collagen(TIC)	Forward	5′-ATGGTGCTCCTGGTGCCA-3′	18
	Reverse	5′-TGTATTCGATGACTGTCTTG-3′	20
Alkaline Phosphatase (ALP)	Forward	5′-AACCTCGAGCAGGAACACAAG-3′	21
	Reverse	5′-CAACGGCAGCAGGAATCC-3′	18
Osteocalcin (OCN)	Forward	5′-ATGAGGACCCTCTCTCTGCTCACT-3′	24
	Reverse	5′-CCCAGCATCATGAGGGCCTGGATCTT-3′	26
Osteopontin (OPN)	Forward	5′-CAACCATGAGACTGGCAGTGGTTTGC-3′	26
	Reverse	5′-GCCTCTTCTTTAATTGACCTCAGAAG-3′	26
Bone Sialoprotein (BSP)	Forward	5′-AGTTGCCAGATTTACCAAACATGA-3′	24
	Reverse	5′-TGTATTAAAAACCAGCCATTGTTCA-3′	26