Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2011 May 15.

Published in final edited form as: Toxicol Appl Pharmacol. 2010 Jan 25;245(1):36–46. doi: 10.1016/j.taap.2010.01.008

Fig. 10 — Effects of acetaminophen on PCNA expression in the liver. Sections were prepared 1–96 h after treatment of wild type (WT) or Cav-1^−/− mice with CTL or acetaminophen (APAP). Upper panel: The number of PCNA-positive hepatocytes was counted in 5 randomly selected microscopic fields (400x), centered on the portal triad. A minimum of 500 hepatocytes were analyzed in each liver section. Data are presented as % PCNA-positive cells. Each bar represents the mean ± SE (n = 6 sections from 3 mice/treatment). ^aSignificantly different (p ≤ 0.05) from CTL; ^bSignificantly different (p ≤ 0.05) from wild type mice. Lower panel: Liver samples from wild type and Cav-1^−/− mice were stained with antibody to PCNA. Antibody binding was visualized by using a Vectastain Elite ABC kit (original magnification, X400). Arrows, PCNA labeled hepatocyte nuclei; arrowheads, PCNA labeled macrophages. One representative section from 3 mice/treatment is shown.

Fig. 10 — Effects of acetaminophen on PCNA expression in the liver. Sections were prepared 1–96 h after treatment of wild type (WT) or Cav-1^−/− mice with CTL or acetaminophen (APAP). Upper panel: The number of PCNA-positive hepatocytes was counted in 5 randomly selected microscopic fields (400x), centered on the portal triad. A minimum of 500 hepatocytes were analyzed in each liver section. Data are presented as % PCNA-positive cells. Each bar represents the mean ± SE (n = 6 sections from 3 mice/treatment). ^aSignificantly different (p ≤ 0.05) from CTL; ^bSignificantly different (p ≤ 0.05) from wild type mice. Lower panel: Liver samples from wild type and Cav-1^−/− mice were stained with antibody to PCNA. Antibody binding was visualized by using a Vectastain Elite ABC kit (original magnification, X400). Arrows, PCNA labeled hepatocyte nuclei; arrowheads, PCNA labeled macrophages. One representative section from 3 mice/treatment is shown.