Effects of loss of Cav-1 on acetaminophen metabolism. Serum was collected 30 min after administration of acetaminophen to wild type and Cav-1−/− mice and analyzed by HPLC for the presence of APAP, APAP-glucuronide (-gluc), and APAP-sulfate (-sulf) metabolites, with retention times of 15, 9.1 and 8 min respectively; IS, internal standard. One representative chromatogram from 3 mice/strain is shown.