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. Author manuscript; available in PMC: 2011 May 1.

Published in final edited form as: Eur Respir J. 2009 Sep 24;35(5):1137–1147. doi: 10.1183/09031936.00085509

The effect of the PI3Kγ inhibitor AS-605240 (AS) on chemokine-induced transmigration was evaluated in vitro. Transmigration across a Transwell filter alone (A) or a confluent layer of cultured murine PECs (B), human A549 cells (C), or human pulmonary microvascular endothelial cells (D) in a Transwell filter was significantly reduced when PMNs were pretreated with AS-605240. No effect was seen when PECs or human pulmonary microvascular endothelial cells were pretreated with the PI3Kγ inhibitor. However, blocking PI3Kγ in A549 cells reduced migration significantly. Data are mean ± SD from 3 separate experiments (each in duplicates). * P < 0.05 versus negative control without chemokine, # P < 0.05 versus positive control without inhibitor.

The effect of the PI3Kγ inhibitor AS-605240 (AS) on chemokine-induced transmigration was evaluated in vitro. Transmigration across a Transwell filter alone (A) or a confluent layer of cultured murine PECs (B), human A549 cells (C), or human pulmonary microvascular endothelial cells (D) in a Transwell filter was significantly reduced when PMNs were pretreated with AS-605240. No effect was seen when PECs or human pulmonary microvascular endothelial cells were pretreated with the PI3Kγ inhibitor. However, blocking PI3Kγ in A549 cells reduced migration significantly. Data are mean ± SD from 3 separate experiments (each in duplicates). * P < 0.05 versus negative control without chemokine, # P < 0.05 versus positive control without inhibitor.