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. 2010 Jan 27;11(2):173–185. doi: 10.1007/s10162-009-0205-8

FIG. 7.

FIG. 7

Serum is required for maximal FSK-induced BrdU incorporation. Cochlear explants were cultured in the absence or presence of FSK in medium containing 10% serum or lacking serum. After 72 h in culture explants were fixed and processed for immunocytochemistry. Bar graph showing the average number of FSK-induced BrdU-labeled nuclei per cochlea from cultured cochlear explants in media with or without serum. The absence of serum significantly reduced the number of FSK-induced BrdU-labeled nuclei (p < 0.001). In serum-containing media (black bars), the average number of BrdU-labeled nuclei per cochlea was 245.8 ± 25.5 for FSK-treated, n = 6, and 1.5 ± 0.7 for untreated (−) FSK, n = 6. In serum-free media (grey bars) the average number of BrdU-labeled nuclei per cochlea was 92.3 ± 16.1 for FSK-treated, n = 6, and 1.8 ± 0.6 for untreated (−) FSK, n = 6.