TABLE 3.
Effects of serum concentration on BrdU labeling density
| Drug condition | Media | No FBS (serum-free) | 1% FBS | 5% FBS | 10% FBS |
|---|---|---|---|---|---|
| No Strept (control) | DMEM | – | 8.21 ± 11.5 | 6.73 ± 12.0 | 5.55 ± 8.87 |
| 3.2% | 2.6% | 2.1% | |||
| BME/EBSS | – | 5.13 ± 9.02 | 4.62 ± 9.31 | 6.16 ± 12.8 | |
| 2.0% | 1.8% | 2.4% | |||
| Plus Strept | DMEMa | 20.2 ± 11.0 7.8% | 17.6 ± 16.2 | 19.1 ± 16.5 | 11.6 ± 10.3 |
| 6.8% | 7.4% | 4.4% | |||
| BME/EBSS | – | 6.33 ± 8.35 | 8.30 ± 14.2 | 6.11 ± 9.06 | |
| 2.4% | 3.2% | 2.4% |
The average number of BrdU-labeled nuclei per 10,000 µm2 ± SD is shown for each type of culture media and each level of serum concentration. In addition, data are also expressed as a percentage of total SCs (assumed to be 260 SCs per 10,000 µm2)
– not analyzed
aThis media type alone showed statistically significant differences across serum concentration by ANOVA (p ≤ 0.05); 0% FBS (no serum, N=7 BPs), 1% FBS, and 5% FBS were significantly different from 10%, but they were not different from one another