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| Day of experiment | Zero | 15–17 | 18 | 20,22 | 30 |
| Phase of carcino- genesis | Initiation | Initiation | Promotion | Initiation | – |
| Procedure | Inoculation | Inocula- tion | Hepatectomy (30% of the liver mass) | Inocula- tion | Sacrifice |
| Substance | DEN | 2AAF | – | 2AAF | – |
| Dose | 300 mg/kg (intra- peritoneal) | 20 mg/kg (gavage) | – | 20 mg/kg (gavage) | – |
DEN, N-nitrosodiethylamine (SIGMA, St Louis, USA); diluted in 0.9% NaCl; 2AAF, 2-acetilaminofluorene (SIGMA, St Louis, USA); diluted in corn oil.
