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. 2008 Jan 10;7(2):177–187. doi: 10.1093/ecam/nem184

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© 2008 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Ashwagandha treatment significantly modulates gene expression in PC-3 cells. Graphical representation of quantitative changes in gene expression by real-time PCR. Relative mRNA species expression was quantitated and expressed as transcript accumulation index (TAI= 2^–ΔΔCT), calculated using the comparative C_T method. All data were controlled for quantity of RNA input by performing measurements on an endogenous reference gene, β-actin. In addition, results on RNA from Ashwagandha-treated samples were normalized to results obtained on RNA from the control, untreated sample. Data are the mean ± SD of three separate experiments done in triplicate. Statistical significance was determined by ANOVA.