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. 2010 May 3;2010:940383. doi: 10.1155/2010/940383

Table 1.

Primer sequences used for the q-RT-PCR.

Gene name Primers sequences Product size (bp)
TLR-2 sense: 5′-GCCTCTCCAAGGAAGAATCC-3′ 144
antisense: 5′-TCCTGTTGTTGGACAGGTCA-3′

TLR-4 sense: 5′-AATCTAGAGCACTTGGACCTTTCC-3′ 116
antisense: 5′-GGGTTCAGGGACAGGTCTAAAGA-3′

TLR-6 sense: 5′-CATCCTATTGTGAGTTTCAGGCAT-3′ 121
antisense: 5′-GCTTCATAGCACTACATCCCAAG-3′

TLR-9 sense: 5′-GGACCTCTGGTACTGCTTCCA-3′ 151
antisense: 5′-AAGCTCGTTGTACACCCAGTCT-3′

β1-defensin sense: 5′-GCCTCTCCCCAGTTCCTGAA-3′ 82
antisense: 5′-GCAGAGAGTAAACAGCAGAAGGTA-3′

β2-defensin sense: 5′-TGTGGTCTCCCTGGAACAAAAT-3′ 105
antisense: 5′-GTCGCACGTCTCTGATGAGG-3′

β3-defensin sense: 5′-CTTCTGTTTGCTTTGCTCTTCCT-3′ 138
antisense: 5′-CTGTTCCTCCTTTGGAAGGCA-3′

β4-defensin sense: 5′-CACTCTACCAACACGCACCTAG-3′ 133
antisense: 5′-CGCAACTGGAACCACACACT-3′

IL-1β sense: 5′-CTGTCCTGCGTGTTGAAAGA-3′ 69
antisense: 5′-TTGGGTAATTTTTGGGATCTACA-3′

IFNγ sense: 5′-GGCATTTTGAAGAATTGGAAAG-3′ 111
antisense: 5′-TTTGGATGCTCTGGTCATCTT-3′

TNFα sense: 5′-CAGCCTCTTCTCCTTCCTGAT-3′ 122
antisense: 5′-GCCAGAGGGCTGATTAGAGA-3′

GAPDH sense: 5′-GGTATCGTCGAAGGACTCATGAC-3′ 180
antisense: 5′-ATGCCAGTGAGCTTCCCGTTCAGC-3′