Table 2.
Kinetic Parameters of the Peptide–Antibody Interaction Measured by Surface Plasmon Resonance
| peptide sequence | kon (M−1 s−1) | koff (s−1) | Kd (M) |
|---|---|---|---|
| CKGLHMWFNC | 3.1 ± 0.1 × 105 | 1.6 ± 0.1 × 10−2 | 5.1 ± 0.1 × 10−8 |
| CAAMGGWSKC | 8.3 ± 0.1 × 103 | 7.3 ± 0.1 × 10−3 | 8.8 ± 0.2 × 10−7 |
| CHLLGGWAKC | 2.8 ± 0.1 × 104 | 6.4 ± 0.1 × 10−2 | 2.3 ± 0.1 × 10−6 |
| CNNPWLAKYC | – | – | 1 × 10−4a |
a
No binding was observed for this peptide at the highest concentration (100 μM) assayed, indicating that its Kd is higher than 10−4 M, the detection limit of the Biacore 3000 instrument.