Fig. 7.
The effect of MeOH and urine matrix in assay buffer. Three different concentrations of 3-PBA standard in each concentration of MeOH or urine solution were mixed with the same volume of phage solution (2.55 × 108 phage particles, 1:4000 dilution in PBST) followed by 2 h incubation on the orbital plate shaker. One hundred microliters of anti-phage MAb-HRP solution in PBST (1:40,000 dilution) was added and incubated for 1 h. Each value represents the mean value of four replicates.