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. Author manuscript; available in PMC: 2010 May 4.
Published in final edited form as: J Am Chem Soc. 2008 Dec 24;130(51):17568–17574. doi: 10.1021/ja807066f

Figure 4.

Figure 4

(A) Synthesis of cross-linker 10. (B) Cross-linking Akt1 and fluorescent substrate peptide 2 with cross-linkers 1 and 10 in the presence or absence of HeLa cell lysate proteins. Akt1, fluorescent peptide 2 with or without HeLa cell lysates, were treated with either cross-linker 1 or 10 for 20 min, followed by SDS-PAGE and in gel fluorescence scanning. (C) Cross-linking of endogenous HeLa cell lysate proteins with substrate peptide 2, in the presence of cross-linker 1 or o-phthaldialdehyde. HeLa cell lysates and substrate peptide 2 were treated with cross-linker 1 or o-phthaldialdehyde for 20 min at r.t., followed by SDS-PAGE and in gel fluorescence scanning.