Figure 1.

³¹P NMR time course experiment of the GSNO and TXPTS reaction. ¹⁵N-GSNO (1 eq.) and TXPTS (2 eq.) were incubated in a HEPES/DTPA buffered solution (pH 7.1) with 10 % D₂O and the reaction was monitored using ³¹P NMR with 1 scan every 20 min. The peaks at 47.2, 39.7, and 34.5 ppm correspond to 5, 3, and 4, respectively and TXPTS starting material at −28.8 ppm. All peaks were referenced to a 3% H₃PO₄ (δ = 0 ppm) external standard which was omitted for clarity.