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. Author manuscript; available in PMC: 2010 May 4.
Published in final edited form as: Probiotics Antimicrob Proteins. 2009 Jan 20;1(1):67–74. doi: 10.1007/s12602-008-9003-6

Fig. 2.

Fig. 2

The effect of lactocin160 and nisin on ATP content of G. vaginalis cells. Open bars represent the level of extracellular ATP, while closed bars represent the total ATP content (extracellular plus the intracellular ATP). Accumulation of extracellular ATP in the G. vaginalis culture treated with lactocin 160 (Lac 160) indicates that the antimicrobial induced the efflux of ATP across the cytoplasmic membrane of the cells (a). In contrast, nisin caused a significant decrease in the total ATP content of the culture, signifying that this bacteriocin triggered the intracellular hydrolysis of ATP in G. vaginalis (b). The corresponding negative controls for Lac 160 and nisin were the cells treated with the lactic acid solution and nisin diluent, respectively (both represented as Control−)