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Neonatal priming with S. Typhi(F1) elicits potent mucosal immunity. Newborn mice were immunized as described in Fig. 2. F1-specific IgA or IgG ASC responses were measured in lung (A) and nasal tissue (B) on days 15 and 70 after birth. Results shown are mean IgA or IgG ASC per 1×106 cells ± SEM of replicate wells. Significant differences (*, p <0.05) compared with the F1 prime-boost group are indicated.
