FIGURE 1.

FoxO3a expression decreases on collagen. A, serum-starved human lung fibroblasts were attached to collagen (100 μg/ml, upper panel)-coated plates as a function of time. Akt and Ser-473-phosphorylated Akt levels were then measured. B, human lung fibroblasts were serum-starved for 2 days followed by attachment to collagen-coated plates as a function of time. FoxO3a, FoxO1, and FoxO4 expression levels were measured (upper and lower panels, respectively). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and actin were used as a loading control. C, serum-starved human lung fibroblasts were attached to fibronectin- (10 μg/ml), vitronectin- (10 μg/ml), and laminin (10 μg/ml)-coated plates as a function of time. FoxO3a expression levels were measured. Actin was used as a loading control.