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. 2010 Mar 5;285(19):14285–14291. doi: 10.1074/jbc.M109.087023

FIGURE 3.

FIGURE 3.

SEM observation of collagen fascicles swollen by the PKD domain. A total of 10 mg of type I insoluble collagen in 1 ml of 20 mm borate buffer (pH 8.5) was incubated at 20 °C with 3 μm EGFP-PKD or EGFP-W36A. The samples were observed using scanning electron microscopy (Hitachi S-570) by Usha and Ramasami's method (23). A, collagen fascicle incubated at 20 °C for 1 h. B, collagen fascicle incubated with W36A-EGFP at 20 °C for 1 h. C, collagen fascicle incubated with EGFP-PKD at 20 °C for 0.5 h. D, collagen fascicle incubated with EGFP-PKD at 20 °C for 1 h. E, diameter of untreated and EGFP-W36A- and EGFP-PKD-treated collagen fascicles. Columns in E show the diameter of: 1) untreated collagen fascicles, 2) EGFP-W36A-treated collagen fascicles, and 3) EGFP-PKD-treated collagen fascicles. All data in E are averages of the data from 50 collagen fascicles measured.