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Activities of chymotrypsin against thermally denatured collagen (1), PKD-untreated collagen (2), and PKD-treated collagen (3). Thermally denatured collagen was prepared by heating insoluble type I collagen at 65 °C for 20 min. PKD-treated collagen was prepared by incubating insoluble type I collagen with 3 μm PKD at 20 °C for 1 h. Chymotrypsin activity was measured at 37 °C by a previously described method (10).
