Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Mar 11;285(19):14178–14186. doi: 10.1074/jbc.M109.094771

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 2. — Product analysis formed from CspLOX2 with 18:2(n-6). 250 μg of 18:2(n-6) were incubated with 15 μg of CspLOX2 for 30 min, and the reaction products were reduced by SnCl₂. A, RP-HPLC-MS² analysis of HODE (top trace, m/z 295 → full scan; peak 1 contained 11-HODE and peak 2 contained HODEs with conjugated double bond systems). B, SP-HPLC analysis of HODEs with conjugated double bond systems. C, MS² spectrum (m/z 295 → full scan) of 11-HODE. D, fragmentation pattern of 11-HODE and resulting fragment sizes.