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. 2010 Mar 11;285(19):14178–14186. doi: 10.1074/jbc.M109.094771

FIGURE 3.

FIGURE 3.

CP-HPLC analysis of the HODE isomers produced by CspLOX2. Product hydroxides were analyzed using a Chiralcel OD-H column (150 × 2.1 mm, 5-μm particle size, Daicel, VWR). A, analysis of 11-HODE methyl ester with a solvent system of n-hexane/2-propanol/trifluoroacetic acid (100:1:0.1, v/v/v) at a flow rate of 0.1 ml/min. B, CP-HPLC analysis of 9-HODE isolated by SP-HPLC separation of the CspLOX2 reaction products with a solvent system of n-hexane/2-propanol/trifluoroacetic acid (100:5:0.1, v/v/v) at a flow rate of 0.1 ml/min. C, CP-HPLC analysis of 13-HODE isolated by SP-HPLC separation of the CspLOX2 reaction products with a solvent system of n-hexane/2-propanol/trifluoroacetic acid (100:5:0.1, v/v/v) at a flow rate of 0.1 ml/min.