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Analysis of the products formed by CspLOX2 incubated with PC-20:4(n-6). Enzyme preparation of CspLOX2 was incubated with PC-20:4(n-6) in 200 mm sodium borate pH 9.5. The reaction products were reduced and transmethylated. A, GC/MS analysis of the trimethylsilyl ether/methyl ester derivatives of the reaction products. B, electron impact mass spectrum of trimethylsilyl ether/methyl ester derivatives of 13-HETE. C, CP-HPLC analysis of conjugated HETE reaction products.
