FIGURE 3.

Dvl requirement for PTH1R activation of β-catenin. A, inhibition of β-catenin activation by the expression of dominant negative Dvl constructs, XDD1 and AHEA-Dvl2. UMR-106 cells were co-transfected with TOPFlash reporter (or the control plasmid, FOPFlash) and either empty vector or the specified Dvl mutant (XDD1 or AHEA-Dvl2). β-Catenin activation was determined from chemiluminescence data as described under “Experimental Procedures.” B, Dvl2-specific shRNA inhibits PTH- and Wnt3A-dependent β-catenin activation in UMR-109 cells. In these experiments, Dvl2 expression was inhibited by co-transfecting two plasmids coding for Dvl2-specific shRNA together with the TOPFlash or FOPFlash reporters.