FIGURE 1.

Effect of WDFY2 depletion on insulin-stimulated glucose uptake. A, 3T3-L1 adipocytes, day 5 of differentiation, were transfected with Scr, WDFY2, or Akt2 siRNA. 48 h after transfection, the cells were serum-starved (3 h), and 2-deoxyglucose uptake was measured after 30 min of insulin stimulation (1 or 100 nm) (n = 3, mean cpm ± S.E. (error bars), p < 0.01, Student's t test). B, WDFY2 and Akt2 mRNA levels were determined by qRT-PCR (n = 3, p < 0.01, Student's t test). C, lysates from A were subjected to SDS-PAGE, and WDFY2 and Akt2 protein levels were analyzed by immunoblotting (IB). Clathrin was used as a loading control.