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Time course of the effect of WDFY2 depletion on Akt2 mRNA and protein levels. A, 3T3-L1 adipocytes transfected with Scr, WDFY2, or Akt2 siRNA were analyzed for the expression of Akt2 by Western blotting 24, 48, and 72 h after transfection (n = 3, representative blot). B, WDFY2 and Akt2 mRNA levels were determined 24, 48, and 72 h after knockdown of WDFY2 using qRT-PCR (n = 3, mean ± S.E. (error bars), p < 0.01, Student's t test).
