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Differential effects of WDFY2 and Akt2 knockdown on mRNA of proteins key to adipocyte function. 3T3-L1 adipocytes were transfected with Scr, WDFY2 (A), Akt2 (B), or Akt1 (C) siRNA on day 4 of differentiation. Samples collected 24, 48, or 72 h after transfection were assayed for mRNA levels of WDFY2, Akt2, and Akt1, respectively, in addition to Glut-4, Glut-1, Cycs, Aco2, nFAS, and SREBP1c using qRT-PCR (n = 4, mean ± S.E. (error bars), p < 0.01, Student's t test).
