FIGURE 5.

Inhibition of RhoA activation in LRP1 gene-silenced Schwann cells decreases cell adhesion and promotes cell migration. LRP1 gene-silenced (A) or GST-RAP-treated (B) Schwann cells were allowed to adhere on FN-coated plates for 1 h in the presence of a specific pharmacological inhibitor for RhoA, Y27632 (25 μm). Data are expressed as the mean ± S.E., n = 3, *, p < 0.01 compared with respective controls. LRP1 gene-silenced (C) or GST-RAP-treated (D) Schwann cells were allowed to migrate in Transwells in the presence of Y27632 for 4 h. Cell migration was expressed as the fold-increase relative to NTC-transfected or GST-treated cells in the absence of inhibitor (mean ± S.E., n = 3, *, p < 0.01 compared with respective control).