TABLE 1.

Inhibition of InhA by PT70 and the INH-NAD adduct

Inhibitor	k2	k−2	t½a	Residence timea	K−1app	Kiapp	koffb	K1c
	min−1	min−1	min	min	nm	nm	min−1	pm
PT70	0.46 ± 0.003	0.041 ± 0.003e	17 ± 1	24 ± 2	62 ± 2	7.8 ± 0.4	0.043 ± 0.006	22 ± 1
PT70	0.46 ± 0.003	0.044 ± 0.008f	17 ± 1	24 ± 2	62 ± 2	7.8 ± 0.4	0.043 ± 0.006	22 ± 1
INH-NADd	0.13 ± 0.01	0.016 ± 0.007	43 ± 12	63 ± 27	100 ± 75	5.0 ± 0.5	0.017 ± 0.001

^a t½ = 0.693/k_off and residence time = 1/k_off, where k_off = k₋₁k₋₂/(k₋₁ + k₂ + k₋₂). Assuming that k₋₁ ≪ k₂ and k₋₂, then k_off ≈ k₋₂.

^b k_off was determined by monitoring the rate of release of ³²P-NAD⁺ from the ternary enzyme·inhibitor complex.

^c K₁ was determined from preincubation experiments.

^d The data were taken from Ref. 5.

^e k₋₂ was determined by progress curve of enzyme·inhibitor complex formation.

^f k₋₂ was determined by progress curve of enzyme activity recovery.