Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
AMPK is required for p53 phosphorylation at Ser46, but not at Ser15, following glucose deprivation. HCT116p53+/+ cells were transfected with pcDNA, pAMPK-WT, or pAMPK-DN expression vectors for 24 h, and then incubated in glucose-depleted medium for 24 h. The phosphorylation level of p53 at Ser15 and Ser46 was analyzed by Western blot. Experiments were repeated three times with similar results, and a representative result is shown.
