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. 2010 Mar 11;285(19):14115–14121. doi: 10.1074/jbc.M110.112672

FIGURE 3.

FIGURE 3.

PP1 mediates direct inhibition of NKCC1. K+ uptake of X. laevis oocytes expressing 15 ng of mutant NKCC1 (F79A, white bars) or wild-type NKCC1 (gray bar) in the presence or absence of 10 ng of wild-type SPAK (SPAK (wt)), wild-type WNK4 (WNK4 (wt)), wild-type PP1 (PP1 (wt)), and constitutively active SPAK (SPAK (ca)) under isosmotic (195 mosmol) conditions. All conditions were repeated twice with similar results. Bars represent mean ± S.E. (n = 20 oocytes). Asterisk denotes statistical significance (p < 0.001). Inset, amino acid sequence of mouse NKCC1 (residues 132–141) with overlapping binding sites for SPAK and PP1 highlighted by horizontal bars.

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