FIGURE 1.

Effect of imatinib on GIST 882 cells. A, cell viability following treatment with medium, DMSO, or 1 μm imatinib was assessed by annexin V-FITC and propidium iodide staining followed by flow cytometric analysis. Viable cells lacked annexin V-FITC, propidium iodide, or both annexin V-FITC and propidium iodide staining. Data are mean (±S.D.) of three experiments. B, Western blot analysis of whole-cell lysates prepared from GIST 882 cells following treatment with medium, DMSO, or 1 μm imatinib. Blots were probed with antibodies specific to a number of both pro- and antiapoptotic factors.