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HPLC analysis of FK506 and FK520 with the retention time of ∼9 and 8 min, respectively. Wild type strain (a), allK in-frame deletion mutant strain (b), allK in-frame deletion mutant strain fed with allylmalonyl-SNAC thioester (compound 10) (c), and the same strain fed with allylmalonyl-diSNAC double thioester (compound 11) (d). Similar incubation conditions and times (given under “Experimental Procedures”) were used for all experiments.
