TABLE 1.

Organization of the all subcluster involved in allyl side chain-providing extender unit of the FK506 gene cluster

Gene No.	Gene name	Proposed function	Start	Stop	Length (amino acids)	Closest characterized homolog
1	allA	Acyltransferase and acyl-carrier protein	2775	4067	431	Homologs of both genes are found in many species of Burkholderia; none of them is characterized (37)
2	allK	Ketoacyl synthase	4078	6465	796
3	allR	Enoyl-CoA reductase	6465	7799	445	Approximately 60% identity to ccr genes from Streptomyces cinnamonensis and Streptomyces collinus (39)
4	allD	Acyl-CoA dehydrogenase	7799	8956	386	32% identical to medium chain acyl-CoA dehydrogenase from Thermus Thermophilus; no close homologs in Streptomyces species (40)
5	allM	Methionine γ lyase	10126	8975	384	38% identical to l-methionine γ-lyase from Citrobacter freundii (42)
6	allN	Transcriptional regulator	10186	10650	155	Homologous to AsnC family of transcriptional regulators present in many bacterial species (33)
7	allP	P450 monooxygenase	10757	11983	409	46% identical to Streptomyces coelicolor A3(2) Cyp154c1, a monooxygenase that functionalizes macrolide ring systems (43)
8	allO	Acyl-CoA oxidoreductase	12171	13358	395	30% identical to 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione hydroxylase from Rhodococcus jostii; belongs to acyl-CoA dehydrogenase (type 2) superfamily (41)
9	allS	Acetoacetyl-CoA reductase or acetoacetyl-ACP reductase	13467	14120	218	45% identical to 3-oxoacyl-[acyl-carrier-protein] reductase from Escherichia fergusonii; also similar to acetoacetyl-CoA reductase from several species (38)