FIGURE 1.
Assembly of bundles of Bs-FtsZ (10 μm) induced by PC (20 μm). A, light scattering time courses with and without PC in Hepes50 assembly buffer, 10 mm MgCl2, pH 6.8, 25 °C. GTP (2 mm) was added at time 0 and 2 mm GDP as indicated by the arrows. B, electron micrograph of Bs-FtsZ with PC. Bar, 200 nm. C, an enlarged bundle. D, its computed diffractogram. The corresponding spacing of the main equatorial spot and the first layer line are indicated in nm. E, Bs-FtsZ polymer pelleting assays at varying pH and ionic strength. F, Bs-FtsZ without PC, magnified as in B.