FIGURE 3.

Bs-FtsZ filament assembly induced by PC. A, solid line, light scattering changes induced on Bs-FtsZ (10 μm) with PC (20 μm) by additions of GMPCPP (25 μm) and MgCl₂ (10 mm) (arrows) in Hepes250, pH 6.8, 25 °C. Dashed line, same without PC. B, example of sedimentation of Bs-FtsZ polymers formed without Mg²⁺ (top) and pellet quantification (bottom). Filled circles, PC and GMPCPP (0.1 mm); filled squares, PC and GTP (2 mm); open symbols, without PC; triangle, PC and GDP; inverted triangle, PC and GMPCP. C, cryoelectron micrograph of Bs-FtsZ filaments assembled with PC and GTP under the same conditions without Mg²⁺. Inset, negatively stained filaments. Bar, 200 nm. D, cryoelectron microscopy filament width distribution, indicating that the filaments are one Bs-FtsZ molecule-wide.