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. 2010 Mar 15;285(19):14450–14458. doi: 10.1074/jbc.M110.109769

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — FLIM-FRET of AKAP79-CFP and AC5-YFP. A, HEK293 cells expressing AKAP79-CFP alone or with PKA RII-YFP or AC5-YFP were imaged in the frequency domain in a wide field FLIM microscope. The lifetime of AKAP79-CFP is significantly decreased when co-expressed with PKA RII-YFP or AC5-YFP, as a result of FRET between CFP and YFP. B, the lifetime of AKAP79ΔB-CFP does not change when co-expressed with AC5-YFP. The graphical quantitation of CFP-tagged protein lifetimes is shown for each panel (mean ± S.E.; n = 3; 60–120 cells). *, p < 0.05.