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. 2010 Feb 18;285(19):14701–14710. doi: 10.1074/jbc.M110.104711

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Endogenous target genes of YT521-B. RT-PCR analysis of YT521-B-dependent exons. Exons were identified by Splicearray analysis. High scoring events were analyzed by RT-PCR using primers in the flanking exons. YT521-B: overexpression of EGFP-YT521-B, YTHdel: overexpression of EGFP-YT521-Bdel(YTH). The image on the right shows a representative RT-PCR analysis, the graph in the middle shows the statistical evaluation of each experiment, and the schematic on the left shows the localization of YTH binding signatures on top of the annotated transcript structure. The processing of GSK3B was shown to be YT521-B independent both by Splicearray analysis and by RT-PCR.