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. Author manuscript; available in PMC: 2010 May 4.
Published in final edited form as: Adv Funct Mater. 2009 Jul 13;19(18):2888–2895. doi: 10.1002/adfm.200900307

Figure 4.

Figure 4

Development and demonstration of an antigen-specific T cell stimulation platform. A) Schematic representation (not to scale) of the completed multicomponent device. The central polymer (black) releases IL-2 and presents anti-CD3 on its surface and the surrounding polymer (gray) releases OVA and presents anti-CD11c. Devices consist of 50 × 50 arrays of 100 μm-diameter regions of the central polymer surrounded by the second polymer. B) Release characteristics of BSA-FITC-loaded polypyrrole (Polypyr) and polypyrrole/poly-1 (Polypyr/Poly-1) films electropolymerized at 0.7V and 1.4V, respectively, with NaDBS as the dopant. BSA-FITC was measured by fluorescence. C) Release characteristics of IL-2-loaded polypyrrole/poly-1 films electropolymerized at 1.4V with NaDBS as the dopant. IL-2 was measured by ELISA (black) and bioactive IL-2 (red) was determined using a splenocyte stimulation assay. D) Fluorescent optical micrograph (FITC and TRITC filters) overlay showing segregation of IgG-AlexaFluor 568 bound to pyrrole/1/2 (1 : 1 : 0.1 ratio) in the central regions and IgG-AlexaFluor 488 bound to 4/acrylic acid (1 : 0.1 ratio) in the periphery. The scale bar represents 100 μm. E) Fluorescent optical micrograph (FITC and TRITC filters) and brightfield overlay of cellular localization of B3Z (stained red) and DC2.4 (green) cells after 72 h incubation with chip described in the text. The array points are polypyrrole/poly-1/poly-2 and the scale bar represents 100 μm. F) IFN-γ release from stimulated B3Z cells measured by ELISA. The Blank Chip consisted of polymers presenting no surface molecules and with no encapsulants that were incubated with cells in the prescence of IL-2 previously treated with OVA. The Chip + IL-2 + OVA was a fully-formed device encapsulating IL-2 and OVA and presenting both anti-CD3 and anti-CD11c. Cells incubated with these devices were not pre-treated with OVA and no exogenous IL-2 was added. Cells Alone were incubated similarly to the other groups but without the presence of a chip, IL-2, or OVA. Cellular stimulation from the loaded and blank chips was statistically significant at the 99.9% confidence level (CL). Error bars represent the mean ± 1 standard deviation.