TABLE 2.
Cytokines detected under various conditions of stimulation
| Cytokine and condition | Amt of cytokine (pg/ml) detected in the indicated group (median [IQR])a |
P valueb | Posthoc testc | ||
|---|---|---|---|---|---|
| Healthy individuals (n = 30) | Patients with pneumonia (n = 30) | Patients with tuberculosis (n = 38) | |||
| IL-6 | |||||
| Unstimulated | 10.7 (10.7, 10.8) A | 10.7 (10.7, 11.3) A | 10.7 (10.7, 11.3) A | 0.131 | NA |
| ESAT-6 | 11.6 (10.8, 13.7) B | 14.1 (11.1, 49.3) B | 41.3 (20.7, 130.9) B | 0.014* | H < T |
| LPS | 175.9 (60.4, 245.7) C | 42.8 (24.1, 172.1) C | 41.5 (20.0, 210.1) B | 0.161 | NA |
| IL-8 | |||||
| Unstimulated | 748.0 (735.0, 760.2) A | 778.4 (748.6, 1100.6) A | 886.2 (751.1, 1645.3) A | 0.003* | H < P = T |
| ESAT-6 | 797.6 (763.1, 997.0) B | 1461.0 (1244.6, 2341.5) B | 3145.8 (1862.9, 4708.9) B | <0.001* | H < T |
| LPS | 1064.6 (899.1, 1920.3) C | 3249.2 (1738.0, 4743.1) C | 2414.3 (1667.4, 5422.0) B | <0.001* | H < P = T |
| IL-10 | |||||
| Unstimulated | 30.2 (28.8, 32.9) A | 31.3 (30.4, 37.8) A | 30.5 (29.2, 32.3) A | 0.869 | NA |
| ESAT-6 | 31.7 (30.0, 34.1) A | 35.8 (31.7, 49.4) B | 35.7 (31.4, 41.4) B | 0.236 | NA |
| LPS | 34.5 (30.8, 39.5) B | 48.1 (36.6, 60.5) C | 37.3 (32.0, 58.1) B | 0.015* | T < P |
| IL-12 | |||||
| Unstimulated | 183.5 (177.9, 196.3) A | 184.9 (180.5, 212.0) A | 190.7 (187.2, 195.4) A | 0.254 | NA |
| ESAT-6 | 184.0 (179.8, 201.1) B | 188.7 (183.2, 212.7) B | 196.0 (188.9, 200.7) B | 0.384 | NA |
| LPS | 199.3 (190.7, 240.7) C | 191.7 (19.4, 237.3) C | 191.3 (187.0, 194.6) A | 0.097 | NA |
| IL-1β | |||||
| Unstimulated | 26.6 (26.5, 27.1) A | 27.0 (26.8, 30.2) A | 26.9 (26.7, 27.8) A | 0.004* | H < P |
| ESAT-6 | 27.8 (26.9, 34.4) B | 30.5 (27.4, 41.1) B | 44.3 (28.9, 58.5) B | 0.470 | NA |
| LPS | 171.5 (100.8, 325.9) C | 82.9 (46.8, 225.7) C | 94.0 (54.8, 287.2) C | 0.735 | NA |
| MCP-1 | |||||
| Unstimulated | 2792.5 (2781.8, 2863.3) A | 3154.6 (2924.7, 3851.7) A | 3458.5 (2940.4, 5197.7) A | 0.001* | H < P = T |
| ESAT-6 | 2804.9 (2796.4, 2985.2) B | 3961.7 (3500.9, 4559.9) B | 5144.7 (4003.3, 6276.0) B | <0.001* | H < P = T |
| LPS | 2831.1 (2805.8, 3575.6) C | 4028.7 (3464.8, 5207.9) B | 4374.1 (3506.4, 5348.4) C | <0.001* | H < T |
| IP-10 | |||||
| Unstimulated | 171.5 (167.6, 183.1) A | 186.4 (178.0, 255.0) A | 182.0 (169.7, 245.8) A | 0.016* | H < T |
| ESAT-6 | 193.0 (175.2, 236.3) B | 215.6 (185.5, 278.2) B | 207.0 (182.0, 309.9) B | 0.125 | NA |
| LPS | 975.0 (402.5, 2716.9) C | 279.6 (207.2, 390.1) C | 266.6 (201.2, 394.1) B | 0.145 | NA |
| TNF-α | |||||
| Unstimulated | 23.6 (22.9, 27.2) A | 23.9 (23.1, 25.5) A | 24.8 (24.2, 25.9) A | 0.712 | NA |
| ESAT-6 | 25.7 (23.4, 30.8) B | 26.5 (23.7, 31.6) B | 28.1 (26.5, 62.6) B | 0.074 | NA |
| LPS | 76.0 (44.5, 158.5) C | 45.3 (28.3, 441.8) C | 35.5 (25.7, 128.8) B | 0.220 | NA |
| IFN-γ | |||||
| Unstimulated | 25.8 (24.9, 27.4) A | 29.0 (27.7, 32.5) A | 27.7 (27.2, 30.1) A | 0.005* | H < T |
| ESAT-6 | 26.0 (25.1, 27.3) AB | 29.3 (27.8, 34.6) A | 30.0 (28.7, 38.5) B | 0.002* | H < T |
| LPS | 27.1 (25.3, 28.6) B | 36.4 (29.7, 43.2) B | 27.9 (26.4, 32.4) A | 0.007* | T < P |
a
Different letters denote significant difference between two stimulations (P < 0.017).
b
Friedman's test, adjusted for age, was used. *, significantly different among the three groups (P < 0.05).
c
Friedman's test, adjusted for age, was used. An adjusted α (α′ = 0.05/3 = 0.017) was applied. P, pneumonia patients; T, tuberculosis patients; H, healthy individuals; NA, not applicable.