TABLE 3.
Percent change of cytokine levels after ESAT-6 and LPS stimulation
| Cytokine and condition | % Change in the indicated group (median [IQR])a |
P valueb | Posthoc testc | ||
|---|---|---|---|---|---|
| Healthy individuals (n = 30) | Patients with pneumonia (n = 30) | Patients with tuberculosis (n = 38) | |||
| IL-6 | |||||
| ESAT-6 | 7.41 (0.14, 27.64) | 22.80 (2.10, 220.86) | 278.58 (91.39, 1063.65) | 0.005* | H = P < T |
| LPS | 1546.64 (458.75, 2180.30) | 275.55 (123.39, 1186.82) | 282.82 (87.05, 1870.94) | 0.161 | NA |
| IL-8 | |||||
| ESAT-6 | 6.93 (1.36, 30.83) | 72.40 (26.01, 122.55) | 147.61 (68.36, 281.16) | 0.001* | H = P < T |
| LPS | 42.90 (20.38, 147.42) | 188.23 (111.04, 335.74) | 127.17 (44.84, 189.81) | 0.011* | H < P |
| IL-10 | |||||
| ESAT-6 | 4.01 (−1.25, 6.89) | 8.06 (1.49, 21.61) | 9.98 (4.92, 27.62) | 0.033* | H < T |
| LPS | 11.29 (2.43, 17.03) | 36.07 (15.89, 75.31) | 18.33 (5.38, 84.51) | 0.002* | H < P |
| IL-12 | |||||
| ESAT-6 | 1.92 (0.53, 4.21) | 1.06 (0.40, 1.87) | 1.06 (0.42, 2.68) | 0.476 | NA |
| LPS | 11.08 (2.92, 26.97) | 5.20 (2.32, 6.80) | 0.11 (−1.62, 1.41) | <0.001* | T < P = H |
| IL-1β | |||||
| ESAT-6 | 2.50 (0.48, 29.41) | 4.45 (0.66, 21.53) | 45.23 (8.98, 111.57) | 0.107 | NA |
| LPS | 5.23 (2.74, 11.21) | 205.15 (36.26, 712.37) | 252.76 (76.70, 778.94) | 0.888 | NA |
| MCP-1 | |||||
| ESAT-6 | 0.76 (0.06, 2.30) | 19.67 (4.69, 26.19) | 23.63 (8.36, 37.14) | 0.005* | H < P = T |
| LPS | 1.41 (0.61, 23.35) | 23.36 (4.18, 40.56) | 5.60 (−0.77, 26.03) | 0.103 | NA |
| IP-10 | |||||
| ESAT-6 | 9.49 (2.68, 38.43) | 4.82 (0.63, 18.40) | 4.19 (1.13, 24.74) | 0.888 | NA |
| LPS | 429.15 (121.25, 1496.97) | 37.76 (13.07, 83.70) | 32.37 (1.60, 95.00) | 0.065 | NA |
| TNF-α | |||||
| ESAT-6 | 3.97 (0.72, 13.77) | 5.47 (2.07, 11.34) | 10.75 (6.97, 114.28) | 0.072 | NA |
| LPS | 231.95 (78.85, 563.07) | 57.04 (11.52, 1475.99) | 25.02 (1.32, 418.69) | 0.097 | NA |
| IFN-γ | |||||
| ESAT-6 | 2.03 (−0.30, 2.71) | 0.73 (−0.03, 1.84) | 7.77 (4.38, 11.71) | <0.001* | H = P < T |
| LPS | 3.59 (1.10, 9.89) | 15.39 (4.03, 36.07) | 0.00 (−4.52, 2.21) | 0.018* | T < P |
a
Percent change was calculated by the following formula: [(value detected after stimulation − value detected in saline)/(value detected in saline)] × 100.
b
Friedman's test, adjusted for age, was used. *, significantly different among the three groups (P < 0.05).
c
Friedman's test, adjusted for age, was used. An adjusted α (α′ = 0.05/3 = 0.017) was applied. P, pneumonia patients; T, tuberculosis patients; H, healthy individuals; NA, not applicable.