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. 2010 Apr;9(4):602–610. doi: 10.1128/EC.00286-09

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Copyright © 2010, American Society for Microbiology.

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Fig. 5. — Vps21 interaction with Vps8 deletion variants. Interactions were tested by two-hybrid analysis. Strain PJ69-4A was transformed with pRK1002 (GAL-DB-VPS21) and a second plasmid coding for one of several different Gal-AD-Vps8Δ fusions. Equal amounts of cells were spotted onto minimal plates containing adenine and histidine (++), only adenine (−his), or only histidine (−ade). Plates were scored after 3 days at 30°C. The transformants contained the following VPS8 plasmids (from left to right): pRK979 (VPS8), pRK1047 (ΔWD40), pRK1033 (ΔPX), pRK1051 (ΔCLH1), pRK1034 (ΔPXA), pRK1045 (ΔCLH2), pRK1035 (ΔEFF), pRK1032 (ΔCLH1+2), pRK1030 (ΔRING), pRK1101 (ΔC-term). Positive controls were pRK329 (GAL-DB vector)/pRK979 (VPS8), pRK1002 (VPS21)/pACT2 (GAL-AD vector), pRK329 + pACT2, and pSE1111 + pSE1112.