Fig. 1.
FOR1, FOR2, and FOR3 increase osmotin resistance of S. cerevisiae cells and are single-copy genes in F. oxysporum f. sp. nicotianae. (A, B, and C) Shown are osmotin sensitivities of cells of S. cerevisiae strain BWG1-7a transformed with p416GPD (vector) or the cDNA clone pFOR1 (A), pFOR2 (B), or pFOR3 (C), which were compared by estimating growth in the presence of various concentrations of osmotin. Growth of cells was measured in liquid culture in selective minimal medium supplemented with the indicated osmotin concentrations. Data were normalized to viable counts of samples without osmotin. Data are the averages ± standard errors (SE) of results from at least three experiments with duplicate samples. (D) Shown are blots of restriction enzyme-digested genomic DNA (15 μg) probed with 32P-labeled FOR1 (lanes 1, 2, and 3), FOR2 (lanes 4, 5, and 6), and FOR3 (lanes 7, 8, and 9). The restriction enzymes used were EcoRI (lanes 1, 4, and 7), PstI (lanes 2, 5, and 8), and XbaI (lanes 3, 6, and 9).