Fig. 3.

FOR2 is the functional homolog of ScGFA1, the gene for GFAT. (A) Cells of S. cerevisiae strain W303-1A and an isogenic Δgfa1::HIS3MX strain transformed with vector (Δgfa1p416GPD) or FOR2 in the same vector (Δgfa1pFOR2) were grown in liquid culture in selective minimal medium supplemented with the indicated osmotin concentrations. Growth was measured as the absorbance at 600 nm and normalized to the absorbance of control cultures grown without osmotin. Data are the averages ± SE of results from two experiments with three independent transformants for each strain. Inset: relative osmotin sensitivities of S. cerevisiae strain W303-1A and the isogenic Δgfa1::HIS3MX strain (Δgfa1) in YPD medium in a similar assay are shown. Data are the averages ± SE of results from two independent experiments. (B) Shown are plates of S. cerevisiae strain JC1246-7A (gfa1-97) transformed with p416GPD or pFOR2, grown for 3 days at 37°C in selective minimal medium without (−) or with (+) glucosamine (1 mg/ml) supplement.