Fig. 6.

Expression and silencing of TbHKT1 in T. brucei. (A) Northern blot with total RNA isolated from cultured T. b. brucei bloodstream forms (BSF) and procyclic forms (PCF). A probe complementary to the 18S rRNA was used as a loading control (left panel). T. b. brucei RNAi cells that express double-stranded TbHKT1 stem-loop constructs under the control of the Tet operator downregulate TbHKT1 expression in the presence of tetracycline (right panel). (B) Growth curves with bloodstream-form (diamonds) and procyclic (triangles) T. b. brucei TbHKT1-RNAi cells; addition of tetracycline (1 mg/ml) to the medium does not impair growth.