Table 1.
Yeast strains used in this study
| Strain | Relevant genotype | Reference |
|---|---|---|
| RH2500 | MATatec1Δ::HIS3 ura3-52 leu2::hisG trp1::hisG | 30 |
| RH2501 | MATatec1Δ::HIS3 ste12Δ::TRP1 ura3-52 leu2::hisG | 30 |
| RH2765 | RH2500 with TCS-CYC1-lacZ::URA3 | 30 |
| RH2767 | RH2500 with CYC1(ΔUAS)-lacZ::URA3 | 30 |
| RH2768 | RH2500 with TCS-PRE-CYC1-lacZ::URA3 | 30 |
| RH2774 | RH2501 with TCS-CYC1-lacZ::URA3 | 30 |
| RH2777 | RH2501 with TCS-PRE-CYC1-lacZ::URA3 | 30 |
| RH2662 | MATaflo11Δ::kanR ura3-52 | 7 |
| EGY48-p1840 | MATα ura3 his3 trp1 leu2 lexAop-lacZ-URA3 | 22 |
| YHUM1631 | RH2765 with PURA3-TEC1::LEU2 | This study |
| YHUM1634 | RH2765 with PURA3-TEC11–280::LEU2 | This study |
| YHUM1635 | RH2765 with PURA3-TEC11–280-TEC1377–486::LEU2 | This study |
| YHUM1640 | RH2774 with PURA3-TEC11–280::LEU2 | This study |
| YHUM1641 | RH2774 with PURA3-TEC11–280-TEC1377–486::LEU2 | This study |
| YHUM1642 | RH2774 with PURA3-TEC11–280-STE121–688::LEU2 | This study |
| YHUM1643 | RH2774 with PURA3-TEC11–280-STE121–250::LEU2 | This study |
| YHUM1644 | RH2774 with PURA3-TEC11–280-STE121–400::LEU2 | This study |
| YHUM1645 | RH2774 with PURA3-TEC11–280-STE12380–688::LEU2 | This study |
| YHUM1646 | RH2774 with PURA3-TEC11–280-STE121–688-TEC1377–486::LEU2 | This study |
| YHUM1647 | RH2774 with PURA3-TEC11–280-STE121–250-TEC1377–486::LEU2 | This study |
| YHUM1648 | RH2774 with PURA3-TEC11–280-STE121–400-TEC1377–486::LEU2 | This study |
| YHUM1649 | RH2774 with PURA3-TEC11–280-STE12380–688-TEC1377–486::LEU2 | This study |
| YHUM1676 | RH2501 with PURA3-TEC1::LEU2 | This study |
| YHUM1694 | RH2500 with PTEC1-TEC1::LEU2 | This study |
| YHUM1696 | RH2500 with tcs(ACATTCcT)-CYC1-lacZ::URA3 | This study |
| YHUM1697 | RH2500 with tcs(ACATTCaT)-CYC1-lacZ::URA3 | This study |
| YHUM1698 | RH2500 with tcs(AtATTCTT)-CYC1-lacZ::URA3 | This study |
| YHUM1699 | RH2500 with tcs(ACATgCTT)-CYC1-lacZ::URA3 | This study |
| YHUM1700 | MATatec1Δ::HIS3 ura3-52 trp1::hisG | This study |
| YHUM1701 | MATatec1Δ::HIS3 ste12Δ::TRP1 ura3-52 | This study |
| YHUM1712 | RH2774 with PTEC1-TEC1::LEU2 | This study |
| YHUM1713 | RH2774 with PURA3-TEC1T273 M::LEU2 | This study |
| YHUM1718 | YHUM1676 with TCS-CYC1-lacZ::URA3 | This study |
| YHUM1720 | YHUM1649 with dig1Δ::kanMX4 | This study |
| YHUM1721 | MATatec1Δ::HIS3 PTEC1-TEC1::LEU2 TCS-CYC1-lacZ::URA3 | This study |
| YHUM1723 | MATatec1Δ::HIS3 PURA3-TEC1T273 M::LEU2 TCS-CYC1-lacZ::URA3 | This study |
| YHUM1724 | MATatec1Δ::HIS3 TCS-CYC1-lacZ::URA3 | This study |
| YHUM1727 | MATatec1Δ::HIS3 PURA3-TEC1::LEU2 TCS-CYC1-lacZ::URA3 | This study |
| YHUM1731 | RH2500 with tcs(ACtTTCTT)-CYC1-lacZ::URA3 | This study |
| YHUM1732 | RH2500 with tcs(ACAgTCTT)-CYC1-lacZ::URA3 | This study |
| YHUM1733 | RH2768 with PURA3-TEC11–280-TEC1377–486::LEU2 | This study |
| YHUM1734 | RH2777 with PURA3-TEC11–280-TEC1377–486::LEU2 | This study |
| YHUM1735 | RH2777 with PURA3-TEC11–280::LEU2 | This study |
| YHUM1736 | RH2768 with PURA3-TEC11–280::LEU2 | This study |
| YHUM1737 | RH2777 with PURA3-TEC11–280-STE121–688-TEC1377–486::LEU2 | This study |
| YHUM1738 | RH2777 with PURA3-TEC11–280-STE12380–688-TEC1377–486::LEU2 | This study |
| YHUM1739 | RH2777 with PURA3-TEC1::LEU2 | This study |
| YHUM1740 | RH2768 with PURA3-TEC1::LEU2 | This study |
| YHUM1743 | YHUM1727 with dig1Δ::kanMX4 | This study |
| YHUM1744 | YHUM1718 with dig1Δ::kanMX4 | This study |
| YHUM1745 | YHUM1644 with dig1Δ::kanMX4 | This study |
| YHUM1746 | YHUM1645 with dig1Δ::kanMX4 | This study |
| YHUM1747 | YHUM1648 with dig1Δ::kanMX4 | This study |
| YHUM1749 | RH2768 with PTEC1-TEC1::LEU2 | This study |
| YHUM1750 | RH2777 with PTEC1-TEC1::LEU2 | This study |
| YHUM1751 | RH2777 with PURA3-TEC11–280-STE121–250-TEC1377–486::LEU2 | This study |
| YHUM1752 | RH2777 with PURA3-TEC11–280-STE121–400-TEC1377–486::LEU2 | This study |
| YHUM1846 | RH2777 with PURA3-TEC11–280-STE121–688::LEU2 | This study |
| YHUM1847 | RH2777 with PURA3-TEC11–280-STE121–250::LEU2 | This study |
| YHUM1848 | RH2777 with PURA3-TEC11–280-STE121–400::LEU2 | This study |
| YHUM1849 | RH2777 with PURA3-TEC11–280-STE12380–688::LEU2 | This study |