Fig. 2.

The dbf-2 mutant is characterized by massive glycogen leakage. (A) Intact hyphal cells are characteristic of wt growth on standard media, while growth of the dbf-2 strain either on solid or in liquid media (middle and right panels, respectively) is accompanied by large cytoplasmic leakage foci at the hyphal tips (arrows). (B) Minimal iodine vapor staining is observed in the wt whereas intense iodine vapor staining is observed in the dbf-2 mutant grown in liquid (upper panel) or on solid (lower panel) media. Arrows indicate iodine vapor staining of leakage foci from hyphal tips of the dbf-2 strain. Images were obtained by light microscopy. (C) The predominant content of the cytoplasmic leakage is glycogen, as determined by ¹H NMR analysis of the droplet material collected from growing dbf-2 mutant hyphal tips, compared with a glycogen standard (upper and lower spectra, respectively).