Fig. 8.

Altered cdc-2 expression in various mutants harboring defects in (dbf-2) Hippo and glycogen pathways. (A and B) Decreased cdc-2-encoded protein levels (detected at 34 and 35 kDa) in dbf-2, ccr-4, mob-1, and mst-1 mutants, compared to the wt and ku-70 control strains, as determined by Western blot analysis (A). A general protein stain demonstrating equal loading is shown as well (B). (C) Changes in cdc-2 expression in different mutants impaired in dbf-2 and glycogen pathway components as determined by real-time RT-PCR. The quantity of cdc-2 cDNA measured was normalized to that of actin cDNA, in extracts from each mutant. Data shown are the average expression levels based on three experiments. Bars indicate standard errors. The abundance of cDNA from the wt strain samples was assigned a value of 1.