Fig. 3.

The protein degradation observed in encysting cells also occurs in homogenates of noninduced trophozoites. (A) Noninduced trophozoites were lysed in a Dounce homogenizer, and the resulting homogenates were incubated at RT under shaking for various periods of time either without any additive or with 0.1% Triton X-100. The asterisks in the acidic, high-molecular-mass region on the gel mark the same proteins as in Fig. 1C. (B) After extended incubation at RT (180 min) with 0.1% Triton X-100, protein fragments that are not observed in encysting cells become apparent (circled).