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. 2010 Apr;9(4):611–618. doi: 10.1128/EC.00300-09

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Copyright © 2010, American Society for Microbiology.

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Fig. 4. — The proteolytic activity observed in trophozoite homogenates resides in the large organellar fraction and can be inhibited with E64. (A) After homogenization, the large organelle fraction was removed from the trophozoite homogenates by centrifugation, and supernatants were incubated at room temperature. Translation elongation factor 2 is marked by an asterisk in panels A and B. (B) The proteolytic activity in trophozoite homogenates can be inhibited only by E64 (30 μM), not by PMSF (3 mM).