Fig. 5.

Swimming-speed diminution in Bug22p-depleted cells. Shown is a track recording of paramecia swimming under a dark-field microscope equipped with a 10× objective during a 650-ms pause. (A) Control Nd7p-depleted cells. (B) Double RNAi targeting all four BUG22 genes. (C and D) Simple RNAi targeting BUG22a and BUG22b, and BUG22c and BUG22d, respectively. Bar = 100 μm. (E) Histograms of measured speeds under all conditions showing quantization of the cumulative effects of double RNAi compared to simple RNAi. The error bars indicate standard errors.