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. 2010 Mar 19;76(9):2884–2894. doi: 10.1128/AEM.02832-09

FIG. 5.

FIG. 5.

Mobilization of stored TAGs by A. borkumensis strains as revealed by TLC analysis. Cells were cultivated in ONR7a medium containing 1% (wt/vol) sodium pyruvate for 72 h at 30°C to promote accumulation of TAGs. Afterwards, cells were harvested, washed twice, and resuspended in carbon-free ONR7a medium to permit the mobilization of TAGs. Samples were taken at the beginning of cultivation (time zero [T0]) and after 24 h (time point 1 [T1]), 48 h (T2), 120 h (T3), and 240 h (T4). Lipid extracts obtained from 5 mg lyophilized cells were applied in each lane. The solvent system used for elution was hexane-diethyl ether-acetic acid (80:20:1, vol/vol/vol), and lipids were visualized by staining with iodine vapor. T, triolein; OA, oleic acid; OO, oleyl oleate; SK2, A. borkumensis wild type; atfA1, A. borkumensis atfA1ΩKm; atfA2, A. borkumensis atfA2ΩSm; atfA1atfA2, A. borkumensis atfA1ΩKm atfA2ΩSm; 2 M131, transposon-induced mutant; FA, fatty acids.